Figure 2 :Histopathology of left sural nerve shows: (a) Features of chronic uniform axonopathy with mild regenerative activity (H and E stain, 350), (b) Higher magnification reveals predominant loss of large myelinated fiber with occasional regenerating cluster (K-pal stain for myelin, 3320), (c) Neurofilament immunostaining for axons highlights the depletion of large diameter axons. Regenerative axonal sprouting is visible as clusters of dot like brown staining of the smaller diameter axons (Neurofilament Immunohistochemistry, 3320)