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ORIGINAL ARTICLE
Year : 2006  |  Volume : 54  |  Issue : 4  |  Page : 402--407

Brain edema after intracerebral hemorrhage in rats: The role of inflammation

Xiangjian Zhang1, Haiyan Li2, Shuchao Hu1, Liying Zhang3, Chunyan Liu1, Chunhua Zhu1, Ruichun Liu1, Chunyan Li1 
1 Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China
2 Department of Neurology, The Beijing Shijitan Hospital, China
3 The Beijing Shijinshan Hospital, China

Correspondence Address:
Chunyan Li
The Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000
China

Background: Intracerebral hemorrhage (ICH) results in secondary brain edema and injury that may lead to death and disability. ICH also causes inflammation. It is unclear whether inflammation contributes to brain edema and neuron injury or functions in repairing the brain tissue. Aims: To understand the effect of inflammation in ICH, we have carried out an investigation on the various aspects and the dynamic changes of inflammation. Settings and Design: An ICH model was generated by injecting 50 ml autologous tail artery blood stereotactically into the right caudate nucleus of 30 rats, which were randomly divided into five ICH groups. Similarly, five Sham control groups were generated by inserting the needle to the right caudate nucleus of rats. Materials and Methods: Rat behavior was evaluated over the time course (6 h, 24 h, 48 h, 72 h and 7 d) in each group. The rats were then killed by administering an overdose of pentobarbital. Following the euthanasia, the brain water content, neuronal loss, glia proliferation, inflammatory infiltration and brain morphology of the rats were measured. Additionally, the expression of TNF-a,IL-6, ICAM-1, VEGF, NF-kB, C3 and CR2 was analyzed by immunohistochemistry. Statistical Analysis: The data were analyzed by student«SQ»s t test. Results: Rat brain water content increased progressively over the time course and reached its peak at 48h followed ICH. The maximum of inflammatory infiltrate (especially neutrophils) and immunopositive cells of TNF-a, IL-6 and NF-kB, were at 48h. The expression of C3 and CR2 reached their peaks at 48-72h, while the expression ICAM-1 and VEGF were at maximum at 72h followed ICH. Conclusions: The results suggested that the inflammatory cytokines, complement system and VEGF may have a function in the development of the brain edema and neuron injury followed ICH.


How to cite this article:
Zhang X, Li H, Hu S, Zhang L, Liu C, Zhu C, Liu R, Li C. Brain edema after intracerebral hemorrhage in rats: The role of inflammation.Neurol India 2006;54:402-407


How to cite this URL:
Zhang X, Li H, Hu S, Zhang L, Liu C, Zhu C, Liu R, Li C. Brain edema after intracerebral hemorrhage in rats: The role of inflammation. Neurol India [serial online] 2006 [cited 2021 Sep 19 ];54:402-407
Available from: https://www.neurologyindia.com/article.asp?issn=0028-3886;year=2006;volume=54;issue=4;spage=402;epage=407;aulast=Zhang;type=0