| ORIGINAL ARTICLE
|Year : 2021 | Volume
| Issue : 6 | Page : 1565--1570
Bone Marrow-Derived Mesenchymal Stem Cells Augment Regeneration of Intervertebral Disc in a Reproducible and Validated Mouse Intervertebral Disc Degeneration Model
Manish Baldia1, Sunithi Mani2, Noel Walter3, Sanjay Kumar4, Alok Srivastava4, Krishna Prabhu1
1 Department of Neurological Sciences, Christian Medical College, Bagayam, Tamil Nadu, India
2 Department of Radiodiagnosis, Christian Medical College, Bagayam, Tamil Nadu, India
3 Department of Pathology, Christian Medical College, Bagayam, Tamil Nadu, India
4 Centre for Stem Cell Research, Christian Medical College, Bagayam, Tamil Nadu, India
Background: Back pain and radicular pain due to disc degeneration are probably the most common problems encountered in neurosurgical practice. The experience and results of stem cell therapy in animal disc degeneration model will help us while doing clinical trials.
Objective: To study the effect of bone marrow-derived mesenchymal stem cells in an established mouse disc degeneration model.
Methods: An easily reproducible mouse coccygeal (Co) 4-5 disc degenerated model by CT-guided percutaneous needle injury was established. The mesenchymal stem cells (MSCs) were cultured from mouse bone marrow and validated. By an established technique, 24 mice disc degenerative models were generated and divided equally into 3 groups (test, placebo, and control). The test group received MSCs with fibrin glue scaffold and placebo group received only scaffold after 6 weeks of degeneration. The control group did not receive any injection. The effects of MSCs were analyzed 8 weeks post injection.
Results: The test group showed a significant change in disc height index (%) in micro CT, whereas in the placebo and control groups, there was no change. The Safranin O staining showed an increase in glycosaminoglycan content and the polarized imaging of picrosirius red staining showed restoration of the collagen fibers in annulus fibrosus, which was statistically significant.
Conclusion: Intradiscal MSC injection restored disc height and promoted regeneration in the discs at the end of 8 weeks. MSC's niche depends on the microenvironment of the host tissue. These findings will be helpful for clinical trials.
Dr. Krishna Prabhu
Professor of Neurosurgery, Department of Neurological Sciences, Christian Medical College, Vellore - 632 004, Tamil Nadu
Source of Support: None, Conflict of Interest: None
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