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| Year : 2001 | Volume
: 49
| Issue : 1 | Page : 78--80 |
The 'hook effect' on serum prolactin estimation in a patient with macroprolactinoma.
AG Unnikrishnan, S Rajaratnam, MS Seshadri, AS Kanagasapabathy, DC Stephen
Department of Endocrinology, Christian Medical College and Hospital, Vellore - 632004, Tamilnadu, India., India
Correspondence Address:
A G Unnikrishnan
Department of Endocrinology, Christian Medical College and Hospital, Vellore - 632004, Tamilnadu, India.
India
 Source of Support: None, Conflict of Interest: None  | Check |
PMID: 11303248 
Large quantities of antigen in an immunoassay system impair antigen-antibody binding, resulting in low antigen determination. This is called the 'high dose hook effect'. We report this phenomenon in a patient with a large macroprolactinoma. In this patient, the correct estimate of serum prolactin (PRL) was obtained only after appropriate dilution of serum. We suggest that in order to avoid the high dose hook effect, the serum PRL be estimated in appropriate dilution in all patients with large pituitary tumours. This is particularly important when the clinical suspicion of high PRL is strong, as in women with amenorrhoea-galactorrhoea and men with long standing hypogonadism.
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